Selected Publications
Gálvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E and Strowig T. 2020. Distinct polysaccharide utilization determines interspecies competition between intestinal Prevotella spp. Cell Host Microbe S1931-3128(20)30515-1.
PubMed Link
Broglia L, Lécrivain AL, Renault TT, Hahnke K, Ahmed-Begrich R, Le Rhun A and Charpentier E. 2020. An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3′-to-5′ exoRNases and RNase Y. Nat. Commun. 11(1):1587.
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Bratovič M, Fonfara I, Chylinski K, Gálvez EJC, Sullivan TJ, Boerno S, Timmermann B, Boettcher M and Charpentier E. 2020. Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches. Nat. Chem. Biol. 16(5):587-595.
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Righetti F, Materne SL, Boss J, Eichner H, Charpentier E and Loh E. 2020. Characterization of a transcriptional TPP riboswitch in the human pathogen Neisseria meningitidis. RNA Biol. 17(5):718-730.
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Makarova KS, Wolf YI, Iranzo J, Shmakov SA, Alkhnbashi OS, Brouns SJJ, Charpentier E, Cheng D, Haft DH, Horvath P, Moineau S, Mojica FJM, Scott D, Shah SA, Siksnys V, Terns MP, Venclovas Č, White MF, Yakunin AF, Yan W, Zhang F, Garrett RA, Backofen R, van der Oost J, Barrangou R and Koonin EV. 2020. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants. Nat. Rev. Microbiol. 18(2):67-83.
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Ratner HK, Escalera-Maurer A, Le Rhun A, Jaggavarapu S, Wozniak JE, Crispell EK, Charpentier E and Weiss DS. 2019. Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence. Mol. Cell 75(3):498-510.
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Davies K and Charpentier E. 2019. Finding her niche: an interview with Emmanuelle Charpentier. CRISPR J. 2:17-22.
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Charpentier E, Elsholz A and Marchfelder A. 2019. CRISPR-Cas: more than 10 years and still full of mysteries. RNA Biol. 16(4):377-379.
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Lander ES, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li J, Liu D, Naldini L, Nie JB, Qiu R, Schoene-Seifert B, Shao F, Terry S, Wei W and Winnacker EL. 2019. Adopt a moratorium on heritable genome editing. Nature 567(7747):165-168.
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Le Rhun A, Escalera-Maurer A, Bratovič M and Charpentier E. 2019. CRISPR-Cas in Streptococcus pyogenes. RNA Biol. 16(4):380-389.
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Lécrivain AL, Le Rhun A, Renault TT, Ahmed-Begrich R, Hahnke K and Charpentier E. 2018. In vivo 3′-to-5′ exoribonuclease targetomes of Streptococcus pyogenes. Proc. Natl. Acad. Sci. U. S. A. 115(46):11814-11819.
PubMed Link
Broglia L, Materne S, Lécrivain AL, Hahnke K, Le Rhun A and Charpentier E. 2018. RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B. RNA Biol. 15(10):1336-1347.
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Tsatsaronis JA, Franch-Arroyo S, Resch U and Charpentier E. 2018. Extracellular Vesicle RNA: A universal mediator of microbial communication? Trends Microbiol. 26(5):401-410.
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Hille F, Richter H, Wong SP, Bratovič M, Ressel S and Charpentier E. 2018. The biology of CRISPR-Cas: Backward and forward. Cell 172(6):1239-1259.
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Charpentier E. 2018. Spotlight on… Emmanuelle Charpentier. FEMS Microbiol. Lett. 365(4).
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Labuhn M, Adams FF, Ng M, Knoess S, Schambach A, Charpentier EM, Schwarzer A, Mateo JL, Klusmann JH and Heckl D. 2018. Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications. Nucleic Acids Res. 46(3):1375-1385.
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Hurwitz JL, Jones BG, Charpentier E and Woodland DL. 2018. Hypothesis: RNA and DNA Viral Sequence Integration into Mammalian Host Genome Supports Long-Term B Cell and T Cell Adaptive Immunity. Viral Immunol. 30(9):628-632.
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Fabiani FD, Renault TT, Peters B, Dietsche T, Gálvez EJC, Guse A, Freier K, Charpentier E, Strowig T, Franz-Wachtel M, Macek B, Wagner S, Hensel M and Erhardt M. 2017. A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum. PLoS Biol. 15(8):e2002267.
PubMed Link
Elsholz AKW, Birk MS, Charpentier E and Turgay K. 2017. Functional diversity of AAA+ protease complexes in Bacillus subtilis. Front. Mol. Biosci. 4:44.
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Reimer J, Knöss S, Labuhn M, Charpentier E, Göhring G, Schlegelberger B, Klusmann JH and Heckl D. 2017. CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Haematologica 102(9):1558-1566.
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Richter F, Fonfara I, Gelfert R, Nack J, Charpentier E and Möglich A. 2017. Switchable Cas9. Curr. Opin. Biotechnol. 48:119-126.
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Renault TT, Abraham AO, Bergmiller T, Paradis G, Rainville S, Charpentier E, Guet CC, Tu Y, Namba K, Keener JP, Minamino T and Erhardt M. 2017. Bacterial flagella grow through an injection-diffusion mechanism. Elife e23136.
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Le Rhun A, Lécrivain AL, Reimegård J, Proux-Wéra E, Broglia L, Della Beffa C and Charpentier E. 2017. Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III in Streptococcus pyogenes. Nucleic Acids Res. 45(5):2329-2340.
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Eggenschwiler R, Moslem M, Fráguas MS, Galla M, Papp O, Naujock M, Fonfara I, Gensch I, Wähner A, Beh-Pajooh A, Mussolino C, Tauscher M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, Cathomen T and Cantz T. 2016. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep. 6:38198.
PubMed Link
Resch U, Tsatsaronis JA, Le Rhun A, Stübiger G, Rohde M, Kasvandik S, Holzmeister S, Tinnefeld P, Wai SN and Charpentier E. 2016. A two-component regulatory system impacts extracellular membrane-derived vesicle production in group A streptococcus. MBio 7(6):e00207-e00216.
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Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier E and Möglich A. 2016. Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Res. 44(20):10003-10014.
PubMed Link
Hille F and Charpentier E. 2016. CRISPR-Cas: biology, mechanisms and relevance. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 371(1707):20150496.
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Labenski V, Suerth JD, Barczak E, Heckl D, Levy C, Bernadin O, Charpentier E, Williams DA, Fehse B, Verhoeyen E and Schambach A. 2016. Alpharetroviral self-inactivating vectors produced by a superinfection-resistant stable packaging cell line allow genetic modification of primary human T lymphocytes. Biomaterials 97:97-109.
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Fonfara I, Richter H, Bratovič M, Le Rhun A and Charpentier E. 2016. The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA. Nature 532(7600):517-521.
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Sternberg SH, Richter H, Charpentier E and Qimron U. 2016. Adaptation in CRISPR-Cas systems. Mol. Cell 61(6):797-808.
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Le Rhun A, Beer YY, Reimegård J, Chylinski K and Charpentier E. 2016. RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes. RNA Biol. 13(2):177-195.
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Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R and Koonin EV. 2015. An updated evolutionary classification of CRISPR-Cas systems. Nat. Rev. Microbiol. 13(11):722-736.
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Charpentier E and Hess WR. 2015. Editorial: RNA in bacteria: biogenesis, regulatory mechanisms and functions. FEMS Microbiol. Rev. 39(3):277-279.
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Heckl D and Charpentier E. 2015. Toward whole-transcriptome editing with CRISPR-Cas9. Mol. Cell 58(4):560-562.
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Charpentier E, Richter H, van der Oost J and White MF. 2015. Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity. FEMS Microbiol. Rev. 39(3):428-441.
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Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna JA, Feng G, Greely HT, Isasi R, Ji W, Kim JS, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS2, Moreno J, Naldini L, Pera M, Perry AC, Venter JC, Zhang F and Zhou Q. 2015. CRISPR germline engineering-the community speaks. Nat. Biotechnol. 33(5):478-486.
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Plagens A, Richter H, Charpentier E and Randau L. 2015. DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes. FEMS Microbiol. Rev. 39(3):442-463.
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Charpentier E. 2015. CRISPR-Cas9: how research on a bacterial RNA-guided mechanism opened new perspectives in biotechnology and biomedicine. EMBO Mol. Med. 7(4):363-365.
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Doudna JA and Charpentier E. 2014. The new frontier of genome engineering with CRISPR-Cas9. Science 346(6213):1258096.
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Latvala S, Mäkelä SM, Miettinen M, Charpentier E and Julkunen I. 2014. Dynamin inhibition interferes with inflammasome activation and cytokine gene expression in Streptococcus pyogenes-infected human macrophages. Clin. Exp. Immunol. 178(2):320-333.
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Charpentier E and Marraffini LA. 2014. Harnessing CRISPR-Cas9 immunity for genetic engineering. Curr. Opin. Microbiol. 19:114-119.
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Charpentier E and Marraffini LA. 2014. Novel technologies in microbiology: Recent advances in techniques in microbiology. Curr. Opin. Microbiol. 19:viii-x.
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Chylinski K, Makarova KS, Charpentier E and Koonin EV. 2014. Classification and evolution of type II CRISPR-Cas systems. Nucleic Acids Res. 42(10):6091-6105.
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Jinek M, Jiang F, Taylor DW, Stenberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E and Doudna JA. 2014. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 343(6176):1247997.
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Fonfara I, Le Rhun A, Chylinski K, Makarova KS, Lécrivain AL, Bzdrenga J, Koonin EV and Charpentier E. 2014. Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems. Nucleic Acids Res. 42(4):2577-2590.
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Fuhrmann J, Mierzwa B, Trentini DB, Spiess S, Lehner A, Charpentier E and Clausen T. 2013. Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in Gram-positive bacteria. Cell Rep. 3(6):1832-1839.
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Chylinski K, Le Rhun A and Charpentier E. 2013. The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems. RNA Biol. 10(5):726-737.
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Charpentier E and Doudna JA. 2013. Biotechnology: Rewriting a genome. Nature 495(7439):50-51.
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Fineran PC and Charpentier E. 2012. Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information. Virology 434(2):202-209.
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Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA and Charpentier E. 2012. A programmable dual-RNA guided DNA endonuclease in adaptive bacterial immunity. Science 337(6096):816-821.
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Le Rhun A and Charpentier E. 2012. Small RNAs in streptococci. RNA Biol. 9(4):414-426.
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Makarova KS, Haft DH, Barrangou R, Brouns SJ, Charpentier E, Horvath P, Moineau S, Mojica FJ, Wolf YI, Yakunin A, van der Oost J and Koonin EV. 2011. Evolution and classification of the CRISPR/Cas systems. Nat. Rev. Microbiol. 9(6):467-477.
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Deltcheva E, Chylinski K, Sharma C, Gonzales K, Chao Y, Pirzada ZA, Eckert M, Vogel J and Charpentier E. 2011. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471(7340):602-607.
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