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Publications

Selected Publications

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Original Articles

  1. Beerens D, Franch-Arroyo S, Sullivan TJ, Goosmann C, Brinkmann V and Charpentier E (2021) Survival Strategies of Streptococcus pyogenes in Response to Phage Infection Viruses, 13(4), 612. DOI: 10.3390/v13040612

  2. Birk MS, Ahmed-Begrich R, Tran S, Elsholz AKW, Frese CK and Charpentier E (2021) Time-Resolved Proteome Analysis of Listeria monocytogenes during Infection Reveals the Role of the AAA+ Chaperone ClpC for Host Cell Adaptation mSystems, 6(4), e0021521. DOI: 10.1128/mSystems.00215-21

  3. Birk MS, Charpentier E and Frese CK (2021) Automated Phosphopeptide Enrichment for Gram-Positive Bacteria J Proteome Res, 20(10), 4886–4892. DOI: 10.1021/acs.jproteome.1c00364

  4. Hüsing S, Halte M, van Look U, Guse A, Gálvez EJC, Charpentier E, Blair DF, Erhardt M and Renault TT (2021) Control of membrane barrier during bacterial type-III protein secretion Nat Commun, 12(1), 3999. DOI: 10.1038/s41467-021-24226-1

  5. Bratovič M, Fonfara I, Chylinski K, Gálvez EJC, Sullivan TJ, Boerno S, Timmermann B, Boettcher M and Charpentier E (2020) Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches Nat Chem Biol, 16(5), 587–595. DOI: 10.1038/s41589-020-0490-4

  6. Broglia L, Lécrivain A-L, Renault TT, Hahnke K, Ahmed-Begrich R, Le Rhun A and Charpentier E (2020) An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3’-to-5’ exoRNases and RNase Y Nat Commun, 11(1), 1587. DOI: 10.1038/s41467-020-15387-6

  7. Gálvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E and Strowig T (2020) Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp Cell Host Microbe, 28(6), 838-852.e6. DOI: 10.1016/j.chom.2020.09.012

  8. Righetti F, Materne SL, Boss J, Eichner H, Charpentier E and Loh E (2020) Characterization of a transcriptional TPP riboswitch in the human pathogen Neisseria meningitidis RNA Biol, 17(5), 718–730. DOI: 10.1080/15476286.2020.1727188

  9. Ratner HK, Escalera-Maurer A, Le Rhun A, Jaggavarapu S, Wozniak JE, Crispell EK, Charpentier E and Weiss DS (2019) Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence Mol Cell, 75(3), 498-510.e5. DOI: 10.1016/j.molcel.2019.05.029

  10. Broglia L, Materne S, Lécrivain A-L, Hahnke K, Le Rhun A and Charpentier E (2018) RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B RNA Biol, 15(10), 1336–1347. DOI: 10.1080/15476286.2018.1532253

  11. Labuhn M, Adams FF, Ng M, Knoess S, Schambach A, Charpentier EM, Schwarzer A, Mateo JL, Klusmann J-H and Heckl D (2018) Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications Nucleic Acids Res, 46(3), 1375–1385. DOI: 10.1093/nar/gkx1268

  12. Lécrivain A-L, Le Rhun A, Renault TT, Ahmed-Begrich R, Hahnke K and Charpentier E (2018) In vivo 3’-to-5’ exoribonuclease targetomes of Streptococcus pyogenes Proc Natl Acad Sci U S A, 115(46), 11814–11819. DOI: 10.1073/pnas.1809663115

  13. Fabiani FD, Renault TT, Peters B, Dietsche T, Gálvez EJC, Guse A, Freier K, Charpentier E, Strowig T, Franz-Wachtel M, Macek B, Wagner S, Hensel M and Erhardt M (2017) A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum PLoS Biol, 15(8), e2002267. DOI: 10.1371/journal.pbio.2002267

  14. Hurwitz JL, Jones BG, Charpentier E and Woodland DL (2017) Hypothesis: RNA and DNA Viral Sequence Integration into the Mammalian Host Genome Supports Long-Term B Cell and T Cell Adaptive Immunity Viral Immunol, 30(9), 628–632. DOI: 10.1089/vim.2017.0099

  15. Le Rhun A, Lécrivain A-L, Reimegård J, Proux-Wéra E, Broglia L, Della Beffa C and Charpentier E (2017) Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III in Streptococcus pyogenes Nucleic Acids Res, 45(5), 2329–2340. DOI: 10.1093/nar/gkw1316

  16. Reimer J, Knöß S, Labuhn M, Charpentier EM, Göhring G, Schlegelberger B, Klusmann J-H and Heckl D (2017) CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo Haematologica, 102(9), 1558–1566. DOI: 10.3324/haematol.2017.164046

  17. Renault TT, Abraham AO, Bergmiller T, Paradis G, Rainville S, Charpentier E, Guet CC, Tu Y, Namba K, Keener JP, Minamino T and Erhardt M (2017) Bacterial flagella grow through an injection-diffusion mechanism Elife, 6, e23136. DOI: 10.7554/eLife.23136

  18. Eggenschwiler R, Moslem M, Fráguas MS, Galla M, Papp O, Naujock M, Fonfara I, Gensch I, Wähner A, Beh-Pajooh A, Mussolino C, Tauscher M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, … Cantz T (2016) Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase Sci Rep, 6, 38198. DOI: 10.1038/srep38198

  19. Fonfara I, Richter H, Bratovič M, Le Rhun A and Charpentier E (2016) The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA Nature, 532(7600), 517–521. DOI: 10.1038/nature17945

  20. Labenski V, Suerth JD, Barczak E, Heckl D, Levy C, Bernadin O, Charpentier E, Williams DA, Fehse B, Verhoeyen E and Schambach A (2016) Alpharetroviral self-inactivating vectors produced by a superinfection-resistant stable packaging cell line allow genetic modification of primary human T lymphocytes Biomaterials, 97, 97–109. DOI: 10.1016/j.biomaterials.2016.04.019

  21. Le Rhun A, Beer YY, Reimegård J, Chylinski K and Charpentier E (2016) RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes RNA Biol, 13(2), 177–195. DOI: 10.1080/15476286.2015.1110674

  22. Resch U, Tsatsaronis JA, Le Rhun A, Stübiger G, Rohde M, Kasvandik S, Holzmeister S, Tinnefeld P, Wai SN and Charpentier E (2016) A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus mBio, 7(6), e00207-16. DOI: 10.1128/mBio.00207-16

  23. Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier E and Möglich A (2016) Engineering of temperature- and light-switchable Cas9 variants Nucleic Acids Res, 44(20), 10003–10014. DOI: 10.1093/nar/gkw930

  24. Chylinski K, Makarova KS, Charpentier E and Koonin EV (2014) Classification and evolution of type II CRISPR-Cas systems Nucleic Acids Res, 42(10), 6091–6105. DOI: 10.1093/nar/gku241

  25. Fonfara I, Le Rhun A, Chylinski K, Makarova KS, Lécrivain A-L, Bzdrenga J, Koonin EV and Charpentier E (2014) Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems Nucleic Acids Res, 42(4), 2577–2590. DOI: 10.1093/nar/gkt1074

  26. Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E and Doudna JA (2014) Structures of Cas9 endonucleases reveal RNA-mediated conformational activation Science, 343(6176), 1247997. DOI: 10.1126/science.1247997

  27. Latvala S, Mäkelä SM, Miettinen M, Charpentier E and Julkunen I (2014) Dynamin inhibition interferes with inflammasome activation and cytokine gene expression in Streptococcus pyogenes-infected human macrophages Clinical and Experimental Immunology, 178(2), 320–333. DOI: 10.1111/cei.12425

  28. Chylinski K, Le Rhun A and Charpentier E (2013) The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems RNA Biol, 10(5), 726–737. DOI: 10.4161/rna.24321

  29. Fuhrmann J, Mierzwa B, Trentini DB, Spiess S, Lehner A, Charpentier E and Clausen T (2013) Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria Cell Rep, 3(6), 1832–1839. DOI: 10.1016/j.celrep.2013.05.023

  30. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA and Charpentier E (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Science, 337(6096), 816–821. DOI: 10.1126/science.1225829

  31. Deltcheva E, Chylinski K, Sharma CM, Gonzales K, Chao Y, Pirzada ZA, Eckert MR, Vogel J and Charpentier E (2011) CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III Nature, 471(7340), 602–607. DOI: 10.1038/nature09886

  32. Fuhrmann J, Schmidt A, Spiess S, Lehner A, Turgay K, Mechtler K, Charpentier E and Clausen T (2009) McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR Science, 324(5932), 1323–1327. DOI: 10.1126/science.1170088

  33. Gratz N, Siller M, Schaljo B, Pirzada ZA, Gattermeier I, Vojtek I, Kirschning CJ, Wagner H, Akira S, Charpentier E and Kovarik P (2008) Group A streptococcus activates type I interferon production and MyD88-dependent signaling without involvement of TLR2, TLR4, and TLR9 J Biol Chem, 283(29), 19879–19887. DOI: 10.1074/jbc.M802848200

  34. Schikora A, Carreri A, Charpentier E and Hirt H (2008) The dark side of the salad: Salmonella typhimurium overcomes the innate immune response of Arabidopsis thaliana and shows an endopathogenic lifestyle PLoS One, 3(5), e2279. DOI: 10.1371/journal.pone.0002279

  35. Siller M, Janapatla RP, Pirzada ZA, Hassler C, Zinkl D and Charpentier E (2008) Functional analysis of the group A streptococcal luxS/AI-2 system in metabolism, adaptation to stress and interaction with host cells BMC Microbiol, 8, 188. DOI: 10.1186/1471-2180-8-188

  36. Vojtek I, Pirzada ZA, Henriques-Normark B, Mastny M, Janapatla RP and Charpentier E (2008) Lysogenic transfer of group A Streptococcus superantigen gene among Streptococci J Infect Dis, 197(2), 225–234. DOI: 10.1086/524687

  37. Garnier F, Janapatla RP, Charpentier E, Masson G, Grélaud C, Stach JF, Denis F and Ploy M-C (2007) Insertion sequence 1515 in the ply gene of a type 1 clinical isolate of Streptococcus pneumoniae abolishes pneumolysin expression J Clin Microbiol, 45(7), 2296–2297. DOI: 10.1128/JCM.02168-06

  38. Charpentier Emmanuelle, Anton AI, Barry P, Alfonso B, Fang Y and Novick RP (2004) Novel cassette-based shuttle vector system for gram-positive bacteria Appl Environ Microbiol, 70(10), 6076–6085. DOI: 10.1128/AEM.70.10.6076-6085.2004

  39. Mangold M, Siller M, Roppenser B, Vlaminckx BJM, Penfound TA, Klein R, Novak R, Novick RP and Charpentier E (2004) Synthesis of group A streptococcal virulence factors is controlled by a regulatory RNA molecule Mol Microbiol, 53(5), 1515–1527. DOI: 10.1111/j.1365-2958.2004.04222.x

  40. Schmitz F-J, Beyer A, Charpentier E, Normark BH, Schade M, Fluit AC, Hafner D and Novak R (2003) Toxin-gene profile heterogeneity among endemic invasive European group A streptococcal isolates J Infect Dis, 188(10), 1578–1586. DOI: 10.1086/379230

  41. Charpentier E., Novak R and Tuomanen E (2000) Regulation of growth inhibition at high temperature, autolysis, transformation and adherence in Streptococcus pneumoniae by clpC Mol Microbiol, 37(4), 717–726. DOI: 10.1046/j.1365-2958.2000.02011.x

  42. Charpentier Emmanuelle, Lavker RM, Acquista E and Cowin P (2000) Plakoglobin Suppresses Epithelial Proliferation and Hair Growth in Vivo Journal of Cell Biology, 149(2), 503–520. DOI: 10.1083/jcb.149.2.503

  43. Novak Rodger, Charpentier E, Braun JS, Park E, Murti S, Tuomanen E and Masure R (2000) Extracellular targeting of choline-binding proteins in Streptococcus pneumoniae by a zinc metalloprotease Molecular Microbiology, 36(2), 366–376. DOI: 10.1046/j.1365-2958.2000.01854.x

  44. Novak Rodger, Charpentier E, Braun JS and Tuomanen E (2000) Signal Transduction by a Death Signal Peptide: Uncovering the Mechanism of Bacterial Killing by Penicillin Molecular Cell, 5(1), 49–57. DOI: 10.1016/S1097-2765(00)80402-5

  45. Novak Rodger, Tuomanen E and Charpentier E (2000) The mystery of psaA and penicillin tolerance in Streptococcus pneumoniae Molecular Microbiology, 36(6), 1505–1506. DOI: 10.1046/j.1365-2958.2000.01959.x

  46. Charpentier Emmanuelle, Gerbaud G and Courvalin P (1999) Conjugative Mobilization of the Rolling-Circle Plasmid pIP823 from Listeria monocytogenes BM4293 among Gram-Positive and Gram-Negative Bacteria Journal of Bacteriology, 181(11), 3368–3374. DOI: 10.1128/jb.181.11.3368-3374.1999

  47. Novak R., Henriques B, Charpentier E, Normark S and Tuomanen E (1999) Emergence of vancomycin tolerance in Streptococcus pneumoniae Nature, 399(6736), 590–593. DOI: 10.1038/21202

  48. Novak Rodger, Cauwels A, Charpentier E and Tuomanen E (1999) Identification of a Streptococcus pneumoniae Gene Locus Encoding Proteins of an ABC Phosphate Transporter and a Two-Component Regulatory System Journal of Bacteriology, 181(4), 1126–1133. DOI: 10.1128/jb.181.4.1126-1133.1999

  49. Novak Rodger, Braun JS, Charpentier E and Tuomanen E (1998) Penicillin tolerance genes of Streptococcus pneumoniae: the ABC-type manganese permease complex Psa Molecular Microbiology, 29(5), 1285–1296. DOI: 10.1046/j.1365-2958.1998.01016.x

  50. Charpentier E and Courvalin P (1997) Emergence of the trimethoprim resistance gene dfrD in Listeria monocytogenes BM4293 Antimicrobial Agents and Chemotherapy, 41(5), 1134–1136. DOI: 10.1128/aac.41.5.1134

  51. Charpentier E., Gerbaud G, Jacquet C, Rocourt J and Courvalin P (1995) Incidence of antibiotic resistance in Listeria species J Infect Dis, 172(1), 277–281. DOI: 10.1093/infdis/172.1.277

  52. Charpentier E, Gerbaud G and Courvalin P (1994) Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis Antimicrobial Agents and Chemotherapy, 38(10), 2330–2335. DOI: 10.1128/aac.38.10.2330

  53. Charpentier Emmanuelle, Gerbaud G and Courvalin P (1993) Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210 Gene, 131(1), 27–34. DOI: 10.1016/0378-1119(93)90665-P

 

Editorials, Opinions & Comments

  1.  Charpentier E, Elsholz A and Marchfelder A (2019) CRISPR-Cas: more than ten years and still full of mysteries RNA Biol, 16(4), 377–379. DOI: 10.1080/15476286.2019.1591659

  2. Davies K and Charpentier E (2019) Finding Her Niche: An Interview with Emmanuelle Charpentier The CRISPR Journal, 2(1), 17–22. DOI: 10.1089/crispr.2019.29042.kda

  3. Lander ES, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li J, Liu D, Naldini L, Nie J-B, Qiu R, Schoene-Seifert B, Shao F, Terry S, Wei W and Winnacker E-L (2019) Adopt a moratorium on heritable genome editing Nature, 567(7747), 165–168. DOI: 10.1038/d41586-019-00726-5

  4. Charpentier E (2018) Spotlight on… Emmanuelle Charpentier FEMS Microbiology Letters, 365(4), fnx271. DOI: 10.1093/femsle/fnx271

  5. Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna J, Feng G, Greely HT, Isasi R, Ji W, Kim J-S, Knoppers B, Lanphier E, Li J, … Zhou Q (2015) CRISPR germline engineering--the community speaks Nat Biotechnol, 33(5), 478–486. DOI: 10.1038/nbt.3227

  6. Charpentier E and Hess WR (2015) Editorial: RNA in bacteria: biogenesis, regulatory mechanisms and functions FEMS Microbiol Rev, 39(3), 277–279. DOI: 10.1093/femsre/fuv025

  7. Charpentier E and Marraffini LA (2014) Editorial overview: Novel technologies in microbiology: Recent advances in techniques in microbiology Curr Opin Microbiol, 19, viii–x. DOI: 10.1016/j.mib.2014.06.012

  8. Charpentier E and Schroeder R (2007) RNA techniques for bacteria Curr Opin Microbiol, 10(3), 254–256. DOI: 10.1016/j.mib.2007.06.003

 

Review Articles

  1. Makarova KS, Wolf YI, Iranzo J, Shmakov SA, Alkhnbashi OS, Brouns SJJ, Charpentier E, Cheng D, Haft DH, Horvath P, Moineau S, Mojica FJM, Scott D, Shah SA, Siksnys V, Terns MP, Venclovas Č, … Koonin EV (2020) Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants Nat Rev Microbiol, 18(2), 67–83. DOI: 10.1038/s41579-019-0299-x

  2. Le Rhun A, Escalera-Maurer A, Bratovič M and Charpentier E (2019) CRISPR-Cas in Streptococcus pyogenes RNA Biol, 16(4), 380–389. DOI: 10.1080/15476286.2019.1582974

  3. Hille F, Richter H, Wong SP, Bratovič M, Ressel S and Charpentier E (2018) The Biology of CRISPR-Cas: Backward and Forward Cell, 172(6), 1239–1259. DOI: 10.1016/j.cell.2017.11.032

  4. Tsatsaronis JA, Franch-Arroyo S, Resch U and Charpentier E (2018) Extracellular Vesicle RNA: A Universal Mediator of Microbial Communication? Trends Microbiol, 26(5), 401–410. DOI: 10.1016/j.tim.2018.02.009

  5. Elsholz AKW, Birk MS, Charpentier E and Turgay K (2017) Functional Diversity of AAA+ Protease Complexes in Bacillus subtilis Front Mol Biosci, 4, 44. DOI: 10.3389/fmolb.2017.00044

  6. Richter F, Fonfara I, Gelfert R, Nack J, Charpentier E and Möglich A (2017) Switchable Cas9 Curr Opin Biotechnol, 48, 119–126. DOI: 10.1016/j.copbio.2017.03.025

  7. Hille F and Charpentier E (2016) CRISPR-Cas: biology, mechanisms and relevance Philos Trans R Soc Lond B Biol Sci, 371(1707), 20150496. DOI: 10.1098/rstb.2015.0496

  8. Sternberg SH, Richter H, Charpentier E and Qimron U (2016) Adaptation in CRISPR-Cas Systems Mol Cell, 61(6), 797–808. DOI: 10.1016/j.molcel.2016.01.030

  9. Charpentier Emmanuelle (2015) CRISPR-Cas9: how research on a bacterial RNA-guided mechanism opened new perspectives in biotechnology and biomedicine EMBO Mol Med, 7(4), 363–365. DOI: 10.15252/emmm.201504847

  10. Charpentier Emmanuelle, Richter H, van der Oost J and White MF (2015) Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity FEMS Microbiol Rev, 39(3), 428–441. DOI: 10.1093/femsre/fuv023

  11. Heckl D and Charpentier E (2015) Toward Whole-Transcriptome Editing with CRISPR-Cas9 Mol Cell, 58(4), 560–562. DOI: 10.1016/j.molcel.2015.05.016

  12. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJM, Terns RM, Terns MP, White MF, Yakunin AF, … Koonin EV (2015) An updated evolutionary classification of CRISPR-Cas systems Nat Rev Microbiol, 13(11), 722–736. DOI: 10.1038/nrmicro3569

  13. Plagens A, Richter H, Charpentier E and Randau L (2015) DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes FEMS Microbiol Rev, 39(3), 442–463. DOI: 10.1093/femsre/fuv019

  14. Charpentier Emmanuelle and Marraffini LA (2014) Harnessing CRISPR-Cas9 immunity for genetic engineering Curr Opin Microbiol, 19, 114–119. DOI: 10.1016/j.mib.2014.07.001

  15. Doudna JA and Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9 Science, 346(6213), 1258096. DOI: 10.1126/science.1258096

  16. Charpentier Emmanuelle and Doudna JA (2013) Biotechnology: Rewriting a genome Nature, 495(7439), 50–51. DOI: 10.1038/495050a

  17. Fineran PC and Charpentier E (2012) Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information Virology, 434(2), 202–209. DOI: 10.1016/j.virol.2012.10.003

  18. Le Rhun A and Charpentier E (2012) Small RNAs in streptococci RNA Biol, 9(4), 414–426. DOI: 10.4161/rna.20104

  19. Makarova KS, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineau S, Mojica FJM, Wolf YI, Yakunin AF, van der Oost J and Koonin EV (2011) Evolution and classification of the CRISPR-Cas systems Nat Rev Microbiol, 9(6), 467–477. DOI: 10.1038/nrmicro2577

  20. Romby P and Charpentier E (2010) An overview of RNAs with regulatory functions in gram-positive bacteria Cell Mol Life Sci, 67(2), 217–237. DOI: 10.1007/s00018-009-0162-8

  21. Charpentier Emmanuelle and Novak R (2000) Bacterial death and antibiotics of the ß-lactam family. Med. Sciences, 16, 1125–1127.

  22. Charpentier Emmanuelle and Tuomanen E (2000) Mechanisms of antibiotic resistance and tolerance in Streptococcus pneumoniae Microbes and Infection, 2(15), 1855–1864. DOI: 10.1016/S1286-4579(00)01345-9

  23. Charpentier E. and Courvalin P (1999) Antibiotic resistance in Listeria spp. Antimicrobial Agents and Chemotherapy, 43(9)(Special).

  24. Charpentier E and Courvalin P (1995) Antibiotic resistance in Listeria spp. Med. Maladies Infect., 25(Special), 225–232.

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Gálvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E and Strowig T. 2020. Distinct polysaccharide utilization determines interspecies competition between intestinal Prevotella spp. Cell Host Microbe S1931-3128(20)30515-1.
PubMed Link

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Broglia L, Lécrivain AL, Renault TT, Hahnke K, Ahmed-Begrich R, Le Rhun A and Charpentier E. 2020. An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3′-to-5′ exoRNases and RNase Y. Nat. Commun. 11(1):1587.
PubMed Link

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Bratovič M, Fonfara I, Chylinski K, Gálvez EJC, Sullivan TJ, Boerno S, Timmermann B, Boettcher M and Charpentier E. 2020. Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches. Nat. Chem. Biol. 16(5):587-595.
PubMed Link

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Righetti F, Materne SL, Boss J, Eichner H, Charpentier E and Loh E. 2020. Characterization of a transcriptional TPP riboswitch in the human pathogen Neisseria meningitidis. RNA Biol. 17(5):718-730.
PubMed Link

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Makarova KS, Wolf YI, Iranzo J, Shmakov SA, Alkhnbashi OS, Brouns SJJ, Charpentier E, Cheng D, Haft DH, Horvath P, Moineau S, Mojica FJM, Scott D, Shah SA, Siksnys V, Terns MP, Venclovas Č, White MF, Yakunin AF, Yan W, Zhang F, Garrett RA, Backofen R, van der Oost J, Barrangou R and Koonin EV. 2020. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants. Nat. Rev. Microbiol. 18(2):67-83.
PubMed Link

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Ratner HK, Escalera-Maurer A, Le Rhun A, Jaggavarapu S, Wozniak JE, Crispell EK, Charpentier E and Weiss DS. 2019. Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence. Mol. Cell 75(3):498-510.
PubMed Link

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Davies K and Charpentier E. 2019. Finding her niche: an interview with Emmanuelle Charpentier. CRISPR J. 2:17-22.
PubMed Link

​

Charpentier E, Elsholz A and Marchfelder A. 2019. CRISPR-Cas: more than 10 years and still full of mysteries. RNA Biol. 16(4):377-379.
PubMed Link

​

Lander ES, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li J, Liu D, Naldini L, Nie JB, Qiu R, Schoene-Seifert B, Shao F, Terry S, Wei W and Winnacker EL. 2019. Adopt a moratorium on heritable genome editing. Nature 567(7747):165-168.
PubMed Link

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Le Rhun A, Escalera-Maurer A, Bratovič M and Charpentier E. 2019. CRISPR-Cas in Streptococcus pyogenes. RNA Biol. 16(4):380-389.
PubMed Link

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Lécrivain AL, Le Rhun A, Renault TT, Ahmed-Begrich R, Hahnke K and Charpentier E. 2018. In vivo 3′-to-5′ exoribonuclease targetomes of Streptococcus pyogenes. Proc. Natl. Acad. Sci. U. S. A. 115(46):11814-11819.
PubMed Link

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Broglia L, Materne S, Lécrivain AL, Hahnke K, Le Rhun A and Charpentier E. 2018. RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B. RNA Biol. 15(10):1336-1347.
PubMed Link

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Tsatsaronis JA, Franch-Arroyo S, Resch U and Charpentier E. 2018. Extracellular Vesicle RNA: A universal mediator of microbial communication? Trends Microbiol. 26(5):401-410.
PubMed Link

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Hille F, Richter H, Wong SP, Bratovič M, Ressel S and Charpentier E. 2018. The biology of CRISPR-Cas: Backward and forward. Cell 172(6):1239-1259.
PubMed Link

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Charpentier E. 2018. Spotlight on… Emmanuelle Charpentier. FEMS Microbiol. Lett. 365(4).
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Labuhn M, Adams FF, Ng M, Knoess S, Schambach A, Charpentier EM, Schwarzer A, Mateo JL, Klusmann JH and Heckl D. 2018. Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications. Nucleic Acids Res. 46(3):1375-1385.
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Hurwitz JL, Jones BG, Charpentier E and Woodland DL. 2018. Hypothesis: RNA and DNA Viral Sequence Integration into Mammalian Host Genome Supports Long-Term B Cell and T Cell Adaptive Immunity. Viral Immunol. 30(9):628-632.
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Fabiani FD, Renault TT, Peters B, Dietsche T, Gálvez EJC, Guse A, Freier K, Charpentier E, Strowig T, Franz-Wachtel M, Macek B, Wagner S, Hensel M and Erhardt M. 2017. A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum. PLoS Biol. 15(8):e2002267.
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Elsholz AKW, Birk MS, Charpentier E and Turgay K. 2017. Functional diversity of AAA+ protease complexes in Bacillus subtilis. Front. Mol. Biosci. 4:44.
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Reimer J, Knöss S, Labuhn M, Charpentier E, Göhring G, Schlegelberger B, Klusmann JH and Heckl D. 2017. CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Haematologica 102(9):1558-1566.
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Richter F, Fonfara I, Gelfert R, Nack J, Charpentier E and Möglich A. 2017. Switchable Cas9. Curr. Opin. Biotechnol. 48:119-126.
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Renault TT, Abraham AO, Bergmiller T, Paradis G, Rainville S, Charpentier E, Guet CC, Tu Y, Namba K, Keener JP, Minamino T and Erhardt M. 2017. Bacterial flagella grow through an injection-diffusion mechanism. Elife e23136.
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Le Rhun A, Lécrivain AL, Reimegård J, Proux-Wéra E, Broglia L, Della Beffa C and Charpentier E. 2017. Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III in Streptococcus pyogenes. Nucleic Acids Res. 45(5):2329-2340.
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Eggenschwiler R, Moslem M, Fráguas MS, Galla M, Papp O, Naujock M, Fonfara I, Gensch I, Wähner A, Beh-Pajooh A, Mussolino C, Tauscher M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, Cathomen T and Cantz T. 2016. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep. 6:38198.
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Resch U, Tsatsaronis JA, Le Rhun A, Stübiger G, Rohde M, Kasvandik S, Holzmeister S, Tinnefeld P, Wai SN and Charpentier E. 2016. A two-component regulatory system impacts extracellular membrane-derived vesicle production in group A streptococcus. MBio 7(6):e00207-e00216.
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Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier E and Möglich A. 2016. Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Res. 44(20):10003-10014.
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Hille F and Charpentier E. 2016. CRISPR-Cas: biology, mechanisms and relevance. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 371(1707):20150496.
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Labenski V, Suerth JD, Barczak E, Heckl D, Levy C, Bernadin O, Charpentier E, Williams DA, Fehse B, Verhoeyen E and Schambach A. 2016. Alpharetroviral self-inactivating vectors produced by a superinfection-resistant stable packaging cell line allow genetic modification of primary human T lymphocytes. Biomaterials 97:97-109.
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Fonfara I, Richter H, Bratovič M, Le Rhun A and Charpentier E. 2016. The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA. Nature 532(7600):517-521.
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Sternberg SH, Richter H, Charpentier E and Qimron U. 2016. Adaptation in CRISPR-Cas systems. Mol. Cell 61(6):797-808.
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Le Rhun A, Beer YY, Reimegård J, Chylinski K and Charpentier E. 2016. RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes. RNA Biol. 13(2):177-195.
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Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R and Koonin EV. 2015. An updated evolutionary classification of CRISPR-Cas systems. Nat. Rev. Microbiol. 13(11):722-736.
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Charpentier E and Hess WR. 2015. Editorial: RNA in bacteria: biogenesis, regulatory mechanisms and functions. FEMS Microbiol. Rev. 39(3):277-279.
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Heckl D and Charpentier E. 2015. Toward whole-transcriptome editing with CRISPR-Cas9. Mol. Cell 58(4):560-562.
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Charpentier E, Richter H, van der Oost J and White MF. 2015. Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity. FEMS Microbiol. Rev. 39(3):428-441.
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Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna JA, Feng G, Greely HT, Isasi R, Ji W, Kim JS, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS2, Moreno J, Naldini L, Pera M, Perry AC, Venter JC, Zhang F and Zhou Q. 2015. CRISPR germline engineering-the community speaks. Nat. Biotechnol. 33(5):478-486.
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Plagens A, Richter H, Charpentier E and Randau L. 2015. DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes. FEMS Microbiol. Rev. 39(3):442-463.
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Charpentier E. 2015. CRISPR-Cas9: how research on a bacterial RNA-guided mechanism opened new perspectives in biotechnology and biomedicine. EMBO Mol. Med. 7(4):363-365.
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Doudna JA and Charpentier E. 2014. The new frontier of genome engineering with CRISPR-Cas9. Science 346(6213):1258096.
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Latvala S, Mäkelä SM, Miettinen M, Charpentier E and Julkunen I. 2014. Dynamin inhibition interferes with inflammasome activation and cytokine gene expression in Streptococcus pyogenes-infected human macrophages. Clin. Exp. Immunol. 178(2):320-333.
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Charpentier E and Marraffini LA. 2014. Harnessing CRISPR-Cas9 immunity for genetic engineering. Curr. Opin. Microbiol. 19:114-119.
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Charpentier E and Marraffini LA. 2014. Novel technologies in microbiology: Recent advances in techniques in microbiology. Curr. Opin. Microbiol. 19:viii-x.
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Chylinski K, Makarova KS, Charpentier E and Koonin EV. 2014. Classification and evolution of type II CRISPR-Cas systems. Nucleic Acids Res. 42(10):6091-6105.
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Jinek M, Jiang F, Taylor DW, Stenberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E and Doudna JA. 2014. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 343(6176):1247997.
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Fonfara I, Le Rhun A, Chylinski K, Makarova KS, Lécrivain AL, Bzdrenga J, Koonin EV and Charpentier E. 2014. Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems. Nucleic Acids Res. 42(4):2577-2590.
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Fuhrmann J, Mierzwa B, Trentini DB, Spiess S, Lehner A, Charpentier E and Clausen T. 2013. Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in Gram-positive bacteria. Cell Rep. 3(6):1832-1839.
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Chylinski K, Le Rhun A and Charpentier E. 2013. The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems. RNA Biol. 10(5):726-737.
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Charpentier E and Doudna JA. 2013. Biotechnology: Rewriting a genome. Nature 495(7439):50-51.
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Fineran PC and Charpentier E. 2012. Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information. Virology 434(2):202-209.
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Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA and Charpentier E. 2012. A programmable dual-RNA guided DNA endonuclease in adaptive bacterial immunity. Science 337(6096):816-821.
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Le Rhun A and Charpentier E. 2012. Small RNAs in streptococci. RNA Biol. 9(4):414-426.
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Makarova KS, Haft DH, Barrangou R, Brouns SJ, Charpentier E, Horvath P, Moineau S, Mojica FJ, Wolf YI, Yakunin A, van der Oost J and Koonin EV. 2011. Evolution and classification of the CRISPR/Cas systems. Nat. Rev. Microbiol. 9(6):467-477.
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Deltcheva E, Chylinski K, Sharma C, Gonzales K, Chao Y, Pirzada ZA, Eckert M, Vogel J and Charpentier E. 2011. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471(7340):602-607.
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